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Gas-tight triblock-copolymer membranes are converted to CO2 permeable by insertion of plant aquaporins

Uehlein, N. and Otto, B. and Eilingsfeld, A. and Itel, F. and Meier, W. and Kaldenhoff, R.. (2012) Gas-tight triblock-copolymer membranes are converted to CO2 permeable by insertion of plant aquaporins. Scientific Reports, 2. p. 1.

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Official URL: http://edoc.unibas.ch/dok/A6070465

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Abstract

We demonstrate that membranes consisting of certain triblock-copolymers were tight for CO2. Using a novel approach, we provide evidence for aquaporin facilitated CO2 diffusion. Plant aquaporins obtained from heterologous expression were inserted into triblock copolymer membranes. These were employed to separate a chamber with a solution maintaining high CO2 concentrations from one with depleted CO2 concentrations. CO2 diffusion was detected by measuring the pH change resulting from membrane CO2 diffusion from one chamber to the other. An up to 21 fold increase in diffusion rate was determined. Besides the supply of this proof of principle, we could provide additional arguments in favour of protein facilitated CO2 diffusion to the vivid on-going debate about the principles of membrane gas diffusion in living cells.
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P. and Itel, Fabian
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Nature Publishing Group
e-ISSN:2045-2322
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:12 Oct 2017 09:35
Deposited On:01 Mar 2013 11:12

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