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Mitochondrial DNA content in paired normal and cancerous breast tissue samples from patients with breast cancer

Fan, Alex Xiu-Cheng and Radpour, Ramin and Haghighi, Mahdi Montazer and Kohler, Corina and Xia, Peng and Hahn, Sinuhe and Holzgreve, Wolfgang and Zhong, Xiao Yan. (2009) Mitochondrial DNA content in paired normal and cancerous breast tissue samples from patients with breast cancer. Journal of cancer research and clinical oncology, Vol. 135. pp. 983-989.

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Official URL: http://edoc.unibas.ch/dok/A6006388

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Abstract

INTRODUCTION: We develop a multiplex quantitative real-time PCR for synchronized analysis of mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) to investigate relative mtDNA abundance in paired normal and cancerous breast tissues. MATERIALS AND METHODS: The amounts of nDNA and mtDNA in 102 tissue samples were quantified for both glyceraldehype-3-phosphodehydrogenase (GAPDH) gene and mtDNA encoded ATPase (MTATP) 8 gene. The average threshold cycle (Ct) number values of the nDNA and mtDNA were used to calculate relative mtDNA content in breast tissues. RESULTS: The median delta Ct (DeltaCt) and the median mtDNA content for normal and cancerous breast tissues were 6.73 and 2.54, as well as 106.50 and 5.80 (P = 0.000, respectively). The mtDNA content was decreased in 82% of cancerous breast tissues compared with the normal ones. The changes were associated with hormone receptor status. CONCLUSION: Our finding suggests that decreased mtDNA content in breast cancer may have diagnostic and prognostic value for the disease.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Gynecological Oncology (Zhong)
UniBasel Contributors:Zhong, Xiao Yan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Springer
ISSN:0084-5353
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:26 Apr 2013 06:59
Deposited On:01 Mar 2013 11:11

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