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Circulating cell-free DNA as a potential biomarker for minimal and mild endometriosis

Zachariah, Rebecca and Schmid, Seraina and Radpour, Ramin and Buerki, Nicole and Fan, Alex Xiu-Cheng and Hahn, Sinuhe and Holzgreve, Wolfgang and Zhong, Xiao Yan. (2009) Circulating cell-free DNA as a potential biomarker for minimal and mild endometriosis. Reproductive biomedicine online : RBM online : an international journal devoted to rapid publication of peer-reviewed biomedical research on human conception and the human embryo, Vol. 18. pp. 407-411.

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Official URL: http://edoc.unibas.ch/dok/A6003258

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Abstract

It has recently been reported that high concentrations of circulating cell-free (ccf) nucleic acids in plasma and serum could be used as biomarkers for non-invasive monitoring a wide variety of malignant and benign proliferations and inflammatory conditions. Endometriosis is one of the most common benign gynaecological proliferations with inflammatory activation in premenopausal women. Real-time multiplex polymerase chain reaction was used for synchronized quantification of the glyceraldehyde-3-phosphate dehydrogenase gene sequence in nuclear DNA (nDNA) and the ATP synthase-8 gene sequence in mitochondrial DNA (mtDNA). DNA was extracted from 500 microl serum and plasma of 19 cases with endometriosis to measure the total amount of ccf nDNA and ccf mtDNA. The concentration of ccf nDNA in plasma was significantly higher in the endometriosis group than in the control group (P = 0.046). The cut-off value selected by a receiver operating characteristic curve could provide a sensitivity of 70% and a specificity of 87% to discriminate between the minimal or mild cases and normal controls. The finding of significantly increased concentrations of ccf nDNA in plasma of patients with endometriosis suggests that ccf nDNA might be a potential biomarker for developing non-invasive diagnostic test in endometriosis.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Gynecological Oncology (Zhong)
UniBasel Contributors:Zhong, Xiao Yan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:1472-6491
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:24 May 2013 09:12
Deposited On:01 Mar 2013 11:11

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