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Tailing and 3'-end labeling of RNA with yeast poly(A) polymerase and various nucleotides

Martin, G. and Keller, W.. (1998) Tailing and 3'-end labeling of RNA with yeast poly(A) polymerase and various nucleotides. RNA, 4 (2). pp. 226-230.

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Official URL: http://edoc.unibas.ch/dok/A5257963

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Abstract

We have tested conditions for the labeling and tailing the 3'-end of RNAs with yeast poly(A) polymerase. Conditions were optimized for addition of NTP, dNTP, or ddNTP nucleotides to RNA. ATP, GTP, and UTP were useful for adding homopolymer tracts of various lengths. The nonradioactive nucleotides biotin-N6-ATP and digoxigenin-11-UTP also were used efficiently.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Keller)
UniBasel Contributors:Keller, Walter
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Cambridge University Press
ISSN:1355-8382
e-ISSN:1469-9001
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:07 Nov 2017 16:19
Deposited On:22 Mar 2012 13:19

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