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Determination of PCR products by CE with contactless conductivity detection

Stojkovic, M. and Uda, N. R. and Brodmann, P. and Popovic, M. and Hauser, P. C.. (2012) Determination of PCR products by CE with contactless conductivity detection. Journal of separation science, Vol. 35, H. 24. pp. 3509-3513.

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Official URL: http://edoc.unibas.ch/dok/A6070766

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Abstract

The use of CE with contactless conductivity detection for the determination of PCR products is demonstrated for the first time. The separation of specific length PCR products according to their size could be achieved using 5% PVP as a sieving medium in a separation buffer consisting of 20 mM Tris and 20 mM 2-(cyclohexylamino)ethansulphonic acid (pH 8.5). A fused silica capillary of 60 cm length and 50 mu m id and an applied separation voltage of 15 kV were employed and separations could be completed within 2050 min. PCR amplified DNA fragments of different sizes obtained from different bacterial plasmid templates as well as a fragment from genomic DNA of genetically modified soybeans could be successfully identified.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
UniBasel Contributors:Hauser, Peter C. P. C.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Wiley-VCH
ISSN:1615-9314
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Mar 2013 11:13
Deposited On:01 Mar 2013 11:09

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