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The Lectin Receptor Kinase-VI.2 is required for priming and positively regulates Arabidopsis pattern-triggered immunity

Singh, P. and Kuo, Y. C. and Mishra, S. and Tsai, C. H. and Chien, C. C. and Chen, C. W. and Desclos-Theveniau, M. and Chu, P. W. and Schulze, B. and Chinchilla, D. and Boller, T. and Zimmerli, L.. (2012) The Lectin Receptor Kinase-VI.2 is required for priming and positively regulates Arabidopsis pattern-triggered immunity. The plant cell, Vol. 24, H. 3. pp. 1256-1270.

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Official URL: http://edoc.unibas.ch/dok/A6070635

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Abstract

Plant cells can be sensitized toward a subsequent pathogen attack by avirulent pathogens or by chemicals such as beta-aminobutyric acid (BABA). This process is called priming. Using a reverse genetic approach in Arabidopsis thaliana, we demonstrate that the BABA-responsive L-type lectin receptor kinase-VI. 2 (LecRK-VI.2) contributes to disease resistance against the hemibiotrophic Pseudomonas syringae and the necrotrophic Pectobacterium carotovorum bacteria. Accordingly, LecRK-VI.2 mRNA levels increased after bacterial inoculation or treatments with microbe-associated molecular patterns (MAMPs). We also show that LecRK-VI.2 is required for full activation of pattern-triggered immunity (PTI); notably, lecrk-VI.2-1 mutants show reduced upregulation of PTI marker genes, impaired callose deposition, and defective stomatal closure. Overexpression studies combined with genome-wide microarray analyses indicate that LecRK-VI.2 positively regulates the PTI response. LecRK-VI.2 is demonstrated to act upstream of mitogen-activated protein kinase signaling, but independently of reactive oxygen production and BOTRYTIS-INDUCED KINASE1 phosphorylation. In addition, complex formation between the MAMP receptor FLAGELLIN SENSING2 and its signaling partner BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 is observed in flg22-treated lecrk-VI.2-1 mutants. LecRK-VI.2 is also required for full BABA-induced resistance and priming of PTI. Our work identifies LecRK-VI.2 as a novel mediator of the Arabidopsis PTI response and provides insight into molecular mechanisms governing priming.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanik > Pflanzenphysiologie Pathogenabwehr (Boller)
UniBasel Contributors:Boller, Thomas and Chinchilla, Delphine
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Society of Plant Biologists
ISSN:1040-4651
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Mar 2013 11:13
Deposited On:01 Mar 2013 11:07

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