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The assembly of CD1e is controlled by an N-terminal propeptide which is processed in endosomal compartments

Maître, Blandine and Angénieux, Catherine and Wurtz, Virginie and Layre, Emilie and Gilleron, Martine and Collmann, Anthony and Mariotti, Sabrina and Mori, Lucia and Fricker, Dominique and Cazenave, Jean-Pierre and van Dorsselaer, Alain and Gachet, Christian and de Libero, Gennaro and Puzo, Germain and Hanau, Daniel and de la Salle, Henri. (2009) The assembly of CD1e is controlled by an N-terminal propeptide which is processed in endosomal compartments. Biochemical journal : BJ, Vol. 419. pp. 661-668.

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Official URL: http://edoc.unibas.ch/dok/A6003265

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Abstract

CD1e displays unique features in comparison with other CD1 proteins. CD1e accumulates in Golgi compartments of immature dendritic cells and is transported directly to lysosomes, where it is cleaved into a soluble form. In these latter compartments, CD1e participates in the processing of glycolipid antigens. In the present study, we show that the N-terminal end of the membrane-associated molecule begins at amino acid 20, whereas the soluble molecule consists of amino acids 32-333. Thus immature CD1e includes an N-terminal propeptide which is cleaved in acidic compartments and so is absent from its mature endosomal form. Mutagenesis experiments demonstrated that the propeptide controls the assembly of the CD1e alpha-chain with beta(2)-microglobulin, whereas propeptide-deleted CD1e molecules are immunologically active. Comparison of CD1e cDNAs from different mammalian species indicates that the CD1e propeptide is conserved during evolution, suggesting that it may also optimize the generation of CD1e molecules in other species.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Experimental Immunology (De Libero)
UniBasel Contributors:De Libero, Gennaro
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Portland Press
ISSN:0264-6021
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Feb 2013 08:46
Deposited On:01 Feb 2013 08:41

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