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Argonaute CLIP - A method to identify in vivo targets of miRNAs

Jaskiewicz, Lukasz and Bilen, Biter and Hausser, Jean and Zavolan, Mihaela. (2012) Argonaute CLIP - A method to identify in vivo targets of miRNAs. Methods, Vol. 58, no. 2. pp. 106-112.

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Official URL: http://edoc.unibas.ch/dok/A6043855

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Abstract

microRNAs are important regulators of gene expression that guide translational repression and degradation of target mRNAs. Only relatively few miRNA targets have been characterized, and computational prediction is hampered by the relatively small number of nucleotides that seem to be involved in target recognition. Argonaute (Ago) crosslinking and immunoprecipitation (CLIP) in combination with next-generation sequencing proved to be a successful method for identifying targets of endogenous cellular miRNAs on a transcriptome-wide scale. Here we review various approaches to Ago CLIP, describe in detail the PAR-CLIP method and provide an outline of the necessary computational analysis for identification of in vivo miRNA binding sites.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Bioinformatics (Zavolan)
UniBasel Contributors:Zavolan, Mihaela
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Academic Press
ISSN:1046-2023
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Oct 2014 09:19
Deposited On:01 Feb 2013 08:39

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