edoc

Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons

Bodmer, Daniel and Levine-Wilkinson, Seamus and Richmond, Alissa and Hirsh, Sarah and Kuruvilla, Rejji. (2009) Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons. Journal of Neuroscience, 29 (23). pp. 7569-7581.

[img]
Preview
PDF - Published Version
Available under License CC BY (Attribution).

3084Kb

Official URL: http://edoc.unibas.ch/dok/A6003900

Downloads: Statistics Overview

Abstract

Nerve growth factor (NGF) is a potent survival and axon growth factor for neuronal populations in the peripheral nervous system. Although the mechanisms by which target-derived NGF influences survival of innervating neurons have been extensively investigated, its regulation of axonal growth and target innervation are just being elucidated. Here, we identify Wnt5a, a member of the Wnt family of secreted growth factors, as a key downstream effector of NGF in mediating axonal branching and growth in developing sympathetic neurons. Wnt5a is robustly expressed in sympathetic neurons when their axons are innervating NGF-expressing targets. NGF:TrkA signaling enhances neuronal expression of Wnt5a. Wnt5a rapidly induces axon branching while it has a long-term effect on promoting axon extension. Loss of Wnt5a function revealed that it is necessary for NGF-dependent axonal branching and growth, but not survival, in vitro. Furthermore, Wnt5a(-/-) mice display reduced innervation of NGF-expressing target tissues, and a subsequent increase in neuronal apoptosis, in vivo. Wnt5a functions in developing sympathetic neurons by locally activating protein kinase C in axons. Together, our findings define a novel regulatory pathway in which Wnt5a, expressed in sympathetic neurons in response to target-derived NGF, regulates innervation of peripheral targets.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Inner Ear Research (Bodmer)
UniBasel Contributors:Bodmer, Daniel K
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Society for Neuroscience
ISSN:0270-6474
e-ISSN:1529-2401
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Related URLs:
Identification Number:
Last Modified:27 Nov 2017 14:43
Deposited On:04 Jan 2013 08:37

Repository Staff Only: item control page