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Automation of image processing in electron crystallography

Arheit, Marcel and Castaño-Díez, Daniel and Thierry, Raphaël and Gipson, Bryant R. and Zeng, Xiangyan and Stahlberg, Henning. (2013) Automation of image processing in electron crystallography. Methods in Molecular Biology, 955. pp. 313-330.

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Official URL: http://edoc.unibas.ch/dok/A6056064

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Abstract

Electron crystallography of membrane proteins records images and diffraction patterns of frozen-hydrated two-dimensional (2D) crystals. To reconstruct the high-resolution three-dimensional (3D) structure of a membrane protein, a multitude of images of 2D crystals have to be processed. Certain processing steps are thereby similar for batches of images that were recorded under similar conditions. Here we describe how the 2dx software package can be used to automate the processing of 2D crystal images, and how the 2D and 3D merging results can be used to iteratively reprocess the images. While the processing of 2D crystal images has been fully automated, the merging process is still semi-manual.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Humana Press
ISSN:1064-3745
e-ISSN:1940-6029
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:12 Oct 2017 10:04
Deposited On:04 Jan 2013 08:37

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