Comparison of the FLOTAC-400 dual technique and the Formalin-Ether Concentration Technique for the diagnosis of human intestinal protozoa infection

Becker, Sören L. and Lohourignon, Laurent K. and Speich, Benjamin and Rinaldi, Laura and Knopp, Stefanie and N'goran, Eliézer K. and Cringoli, Giuseppe and Utzinger, Jürg. (2011) Comparison of the FLOTAC-400 dual technique and the Formalin-Ether Concentration Technique for the diagnosis of human intestinal protozoa infection. Journal of clinical microbiology, Vol. 49, H. 6. pp. 2183-2190.

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Official URL: http://edoc.unibas.ch/dok/A6002147

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There is a need for accurate diagnosis of intestinal parasite infections in humans, but currently available copromicroscopic techniques have shortcomings, such as low sensitivity. We compared the diagnostic accuracy of a further modified version of the recently developed FLOTAC technique with the widely used formalin-ether concentration technique (FECT) for the detection of intestinal protozoa in human stool samples. Formaldehyde-preserved stool samples from 108 individuals in Cote d'Ivoire were subjected to the FLOTAC-400 dual technique using two different flotation solutions (FS), and the FECT. Stool samples were examined according to computer-generate random lists by an experienced laboratory technician blinded for the results of each method. Both methods detected the same eight intestinal protozoa species. Whilst the FLOTAC-400 dual technique (results from both FS combined) found higher prevalences of Entamoeba coli (77.8% vs. 71.3%, p>0.001), Blastocystis hominis (20.4% vs. 13.0%, p=0.458) and Giardia intestinalis (8.3% vs. 6.5%, p>0.001), the FECT detected higher prevalences of Entamoeba histolytica/E. degrees dispar (27.8% vs. 20.4%, p=0.019) and four species of non-pathogenic intestinal protozoa. The diagnostic agreement between the two methods differed considerably depending on the intestinal protozoa investigated (kappa measures; range, 0.01-0.46). Our study confirmed that the FLOTAC-400 dual technique can be utilized for diagnosis of intestinal protozoa infections in humans. Since FLOTAC is a sensitive technique for the detection of soil-transmitted helminths and Schistosoma mansoni, it might become a viable copromicroscopic technique for the concurrent diagnosis of helminths and intestinal protozoa infections
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Former Units within Swiss TPH > Health Impact Assessment (Utzinger)
UniBasel Contributors:Utzinger, Jürg
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:07 Aug 2015 12:06
Deposited On:08 Nov 2012 16:20

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