Engineered synthetic virus-like particles and their use in vaccine delivery

Ghasparian, A. and Riedel, T. and Koomullil, J. and Moehle, K. and Gorba, C. and Svergun, D. I. and Perriman, A. W. and Mann, S. and Tamborrini, M. and Pluschke, G. and Robinson, J. A.. (2011) Engineered synthetic virus-like particles and their use in vaccine delivery. ChemBioChem : a European journal of chemical biology, Vol. 12, H. 1. pp. 100-109.

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Official URL: http://edoc.unibas.ch/dok/A6002187

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Engineered nanoparticles have been designed based on the self-assembling properties of synthetic coiled-coil lipopeptide building blocks. The presence of an isoleucine zipper within the lipopeptide together with the aggregating effects of an N-terminal lipid drives formation of 20-25 nm nanoparticles in solution. Biophysical studies support a model in which the lipid is buried in the centre of the nanoparticle, with 20-30 trimeric helical coiled-coil bundles radiating out into solution. A promiscuous T-helper epitope and a synthetic B-cell epitope mimetic derived from the circumsporozoite protein of Plasmodium falciparum have been linked to each lipopeptide chain, with the result that 60-90 copies of each antigen are displayed over the surface of the nanoparticle. These nanoparticles elicit strong humoral immune responses in mice and rabbits, including antibodies able to cross-react with the parasite, thereby, supporting the potential value of this delivery system in synthetic vaccine design
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Molecular Immunology (Pluschke)
UniBasel Contributors:Pluschke, Gerd and Tamborrini, Marco
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:08 Nov 2012 16:23
Deposited On:08 Nov 2012 16:19

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