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Imaging streptavidin 2D crystals on biotinylated lipid monolayers at high resolution with the atomic force microscope

Scheuring, S. and Muller, D. J. and Ringler, P. and Heymann, J. B. and Engel, A.. (1999) Imaging streptavidin 2D crystals on biotinylated lipid monolayers at high resolution with the atomic force microscope. Journal of Microscopy, Vol. 193, H. 1. pp. 28-35.

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Official URL: http://edoc.unibas.ch/dok/A5257671

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Abstract

Streptavidin crystals were grown on biotinylated lipid monolayers at an air/water interface and transferred onto highly oriented pyrolytic graphite (HOPG). These arrays could be imaged to a resolution below 1 nm using the atomic force microscope. The surface topographs obtained were compared with negative-stain electron microscopy images and the atomic model as determined by X-ray crystallography. The streptavidin tetramer (60 kDa) exposes two free biotin-binding sites to the buffer solution, while two are occupied by linkage to the lipid monolayer. Therefore, the streptavidin 2D crystals can be used as nanoscale matrices for binding biotinylated compounds. Furthermore, this HOPG-based preparation method provides a general novel approach to study the structure of protein arrays assembled on lipid monolayers with the AFM.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
UniBasel Contributors:Engel, Andreas H
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Blackwell Scientific Publications
ISSN:0022-2720
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:20
Deposited On:22 Mar 2012 13:18

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