Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors

Braun, T. and Backmann, N. and Vogtli, M. and Bietsch, A. and Engel, A. and Lang, H. -P. and Gerber, C. and Hegner, M.. (2006) Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors. Biophysical journal, Vol. 90, H. 8. pp. 2970-2977.

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Official URL: http://edoc.unibas.ch/dok/A5257631

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Bacteriorhodopsin proteoliposomes were used as a model system to explore the applicability of micromechanical cantilever arrays to detect conformational changes in membrane protein patches. The three main results of our study concern: 1), reliable functionalization of micromechanical cantilever arrays with proteoliposomes using ink jet spotting; 2), successful detection of the prosthetic retinal removal (bleaching) from the bacteriorhodopsin protein by measuring the induced nanomechanical surface stress change; and 3), the quantitative response thereof, which depends linearly on the amount of removed retinal. Our results show this technique to be a potential tool to measure membrane protein-based receptor-ligand interactions and conformational changes.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
UniBasel Contributors:Engel, Andreas H
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Biophysical Society
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:20
Deposited On:22 Mar 2012 13:18

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