Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors

Braun, Thomas and Backmann, Natalija and Vogtli, Manuel and Bietsch, Alexander and Engel, Andreas and Lang, Hans-Peter and Gerber, Christoph and Hegner, Martin. (2006) Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors. Biophysical journal, 90 (8). pp. 2970-2977.

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Official URL: http://edoc.unibas.ch/dok/A5257631

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Bacteriorhodopsin proteoliposomes were used as a model system to explore the applicability of micromechanical cantilever arrays to detect conformational changes in membrane protein patches. The three main results of our study concern: 1), reliable functionalization of micromechanical cantilever arrays with proteoliposomes using ink jet spotting; 2), successful detection of the prosthetic retinal removal (bleaching) from the bacteriorhodopsin protein by measuring the induced nanomechanical surface stress change; and 3), the quantitative response thereof, which depends linearly on the amount of removed retinal. Our results show this technique to be a potential tool to measure membrane protein-based receptor-ligand interactions and conformational changes.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Braun)
UniBasel Contributors:Engel, Andreas H and Braun, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Biophysical Society
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Oct 2021 10:13
Deposited On:22 Mar 2012 13:18

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