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Malaria vaccine candidate : design of a multivalent subunit alpha-helical coiled coil poly-epitope

Olugbile, S. and Villard, V. and Bertholet, S. and Jafarshad, A. and Kulangara, C. and Roussilhon, C. and Frank, G. and Agak, G. W. and Felger, I. and Nebie, I. and Konate, K. and Kajava, A. V. and Schuck, P. and Druilhe, P. and Spertini, F. and Corradin, G.. (2011) Malaria vaccine candidate : design of a multivalent subunit alpha-helical coiled coil poly-epitope. Vaccine : the official journal of the International Society for Vaccines, 29 (40). pp. 7090-7099.

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Official URL: http://edoc.unibas.ch/dok/A6002267

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Abstract

A new strategy for the rapid identification of new malaria antigens based on protein structural motifs was previously described. We identified and evaluated the malaria vaccine potential of fragments of several malaria antigens containing alpha-helical coiled coil protein motifs. By taking advantage of the relatively short size of these structural fragments, we constructed different poly-epitopes in which 3 or 4 of these segments were joined together via a non-immunogenic linker. Only peptides that are targets of human antibodies with anti-parasite in vitro biological activitieswere incorporated. One of the constructs, P181, was well recognized by sera and peripheral blood mononuclear cells (PBMC) of adults living in malaria-endemic areas. Affinity purified antigen-specific human antibodies and sera from P181-immunized mice recognised native proteins on malaria-infected erythrocytes in both immunofluorescence and western blot assays. In addition, specific antibodiesinhibited parasite development in anantibody dependent cellular inhibition (ADCI) assay. Naturally induced antigen-specific human antibodies were at high titers and associated with clinical protection from malaria in longitudinal follow-up studies in Senegal
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Molecular Diagnostics (Felger)
UniBasel Contributors:Felger, Ingrid
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0264-410X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:18 Apr 2018 07:59
Deposited On:08 Nov 2012 16:08

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