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Quantification of plasma lactate concentrations using capillary electrophoresis with contactless conductivity detection

Pormsila, Worapan and Morand, Réjane and Krähenbühl, Stephan and Hauser, Peter C.. (2011) Quantification of plasma lactate concentrations using capillary electrophoresis with contactless conductivity detection. Electrophoresis, 32 (8). pp. 884-889.

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Official URL: http://edoc.unibas.ch/dok/A6001928

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Abstract

The quantification of plasma lactate and evaluation of the lactate threshold by CE with capacitively coupled contactless conductivity is demonstrated. The only sample preparation needed was deproteinization with a ACN/methanol mixture. A solution of 10 mmol/L 2-morpholinoethanesulfonic acid monohydrate, 10 mmol/L DL-histidine, 70 mu mol/L hexadecyltrimethylammonium bromide, pH 6.0 was found suitable as running buffer. Linearity was achieved for the concentration range of 10-1000 mu mol/L with a correlation coefficient of 0.9994. The limit of detection (3 S/N) was determined as 3.2 mu mol/L. Intra-and inter-day variabilities were less than 7% RSD. The suitability of the method could be demonstrated by analyzing various clinical samples, where the results correlated satisfactorily with those of an established enzymatic method.
Faculties and Departments:03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie > Klinische Pharmakologie (Krähenbühl)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie > Klinische Pharmakologie (Krähenbühl)
03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Clinical Pharmacology (Krähenbühl)
05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmakologie (Krähenbühl)
UniBasel Contributors:Hauser, Peter C. P. C. and Morand, Réjane and Krähenbühl, Stephan
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:0173-0835
e-ISSN:1522-2683
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:29 Mar 2017 13:17
Deposited On:11 Oct 2012 15:27

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