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Capillary electrophoresis with contactless conductivity detection for the determination of carnitine and acylcarnitines in clinical samples

Pormsila, Worapan and Morand, Réjane and Krähenbühl, Stephan and Hauser, Peter C.. (2011) Capillary electrophoresis with contactless conductivity detection for the determination of carnitine and acylcarnitines in clinical samples. Journal of Chromatography B, 879 (13-14). pp. 921-926.

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Official URL: http://edoc.unibas.ch/dok/A6001927

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Abstract

A capillary electrophoresis method with contactless conductivity detection was developed for the quantification of carnitine and six acylcarnitines in plasma and urine samples. The running buffer employed consisted of 500 mmol/L acetic acid, 1.0 mmol/L hydroxypropyl-beta-cyclodextrin and 0.05% Tween at a pH of 2.6. Under these conditions, the isomeric valproyl- and octanoyl-carnitines could be distinguished. The linearity was in the range from 5.0 to 200.0 mu mol/L with correlation coefficients between 0.9992 and 0.9997. The limits of detection were between 1.0 and 3.2 mu mol/L Intra- and inter-day precisions as %RSD were better than 10%. The method allows for direct determination without derivatisation or extraction processes. The method was applied for the quantification of carnitine and acetylcarnitine in plasma pre- and post-exercise, and to measure valproylcarnitine in plasma and urine of patients undergoing valproate therapy. (C) 2011 Elsevier B.V. All rights reserved.
Faculties and Departments:03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie > Klinische Pharmakologie (Krähenbühl)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie > Klinische Pharmakologie (Krähenbühl)
03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Clinical Pharmacology (Krähenbühl)
05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmakologie (Krähenbühl)
UniBasel Contributors:Hauser, Peter C. P. C. and Morand, Réjane and Krähenbühl, Stephan
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:1570-0232
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:29 Mar 2017 13:16
Deposited On:11 Oct 2012 15:25

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