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New polymorphic microsatellite markers of the endangered meadow viper (; Vipera ursinii; ) identified by 454 high-throughput sequencing: when innovation meets conservation

Metzger, Cesar and Ferchaud, Anne-Laure and Geiser, Celine and Ursenbacher, Sylvain. (2011) New polymorphic microsatellite markers of the endangered meadow viper (; Vipera ursinii; ) identified by 454 high-throughput sequencing: when innovation meets conservation. Conservation genetics resources, 3 (3). pp. 589-592.

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Official URL: http://edoc.unibas.ch/dok/A6001706

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Abstract

The Next Generation Sequencing (pyrose- quencing) technique allows rapid, low-cost development of microsatellite markers. We have used this technology to develop 14 polymorphic loci for the endangered meadow viper (Vipera ursinii). Based on 37,000 reads, we devel- oped primers for 66 microsatellite loci and found that 14 were polymorphic. The number of alleles per locus varies from 1 to 12 (for 30 individuals tested). At a cost of about 1/3 that of a normal microsatellite development, we were able to define enough microsatellite markers to conduct population genetic studies on a non-model species.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Integrative Biologie > Naturschutzbiologie (Baur)
UniBasel Contributors:Ursenbacher, Sylvain
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Springer
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:20 Sep 2017 14:26
Deposited On:11 Oct 2012 15:14

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