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Automated screening of 2D crystallization trials using transmission electron microscopy : a high-throughput tool-chain for sample preparation and microscopic analysis

Coudray, N. and Hermann, G. and Caujolle-Bert, D. and Karathanou, A. and Erne-Brand, F. and Buessler, J. L. and Daum, P. and Plitzko, J. M. and Chami, M. and Mueller, U. and Kihl, H. and Urban, J. P. and Engel, A. and Remigy, H. W.. (2011) Automated screening of 2D crystallization trials using transmission electron microscopy : a high-throughput tool-chain for sample preparation and microscopic analysis. Journal of structural biology, Vol. 173, H. 2. pp. 365-374.

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Official URL: http://edoc.unibas.ch/dok/A6002460

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Abstract

We have built and extensively tested a tool-chain to prepare and screen two-dimensional crystals of membrane proteins by transmission electron microscopy (TEM) at room temperature. This automated process is an extension of a new procedure described recently that allows membrane protein 2D crystallization in parallel (Iacovache et al., 2010). The system includes a gantry robot that transfers and prepares the crystalline solutions on grids suitable for TEM analysis and an entirely automated microscope that can analyze 96 grids at once without human interference. The operation of the system at the user level is solely controlled within the MATLAB environment: the commands to perform sample handling (loading/unloading in the microscope), microscope steering (magnification, focus, image acquisition, etc.) as well as automatic crystal detection have been implemented. Different types of thin samples can efficiently be screened provided that the particular detection algorithm is adapted to the specific task. Hence, operating time can be shared between multiple users. This is a major step towards the integration of transmission electron microscopy into a high throughput work-flow.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
UniBasel Contributors:Engel, Andreas H
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Academic Press
ISSN:1047-8477
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:13 Sep 2013 07:50
Deposited On:14 Sep 2012 07:15

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