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A MAS NMR study of the bacterial ABC transporter ArtMP

Lange, Vivien and Becker-Baldus, Johanna and Kunert, Britta and van Rossum, Barth-Jan and Casagrande, Fabio and Engel, Andreas and Roske, Yvette and Scheffel, Frank M. and Schneider, Erwin and Oschkinat, Hartmut. (2010) A MAS NMR study of the bacterial ABC transporter ArtMP. ChemBioChem, Vol. 11, H. 4. pp. 547-555.

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Official URL: http://edoc.unibas.ch/dok/A6002471

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Abstract

ATP-binding cassette (ABC) transport systems facilitate the translocation of substances, like amino acids, across cell membranes energised by ATP hydrolysis. This work describes first structural studies on the ABC transporter ArtMP from Geobacillus stearothermophilus in native lipid environment by magic-angle spinning NMR spectroscopy. The 2D crystals of ArtMP and 3D crystals of isolated ArtP were prepd. in different nucleotide-bound or -unbound states. From selectively 13C,15N-labeled ArtP, several sequence-specific assignments were obtained, most of which could be transferred to spectra of ArtMP. Residues Tyr133 and Pro134 protrude directly into the ATP-binding pocket at the interface of the ArtP subunits, and hence, are sensitive monitors for structural changes during nucleotide binding and hydrolysis. Distinct sets of NMR shifts were obtained for ArtP with different phosphorylation states of the ligand. Indications were found for an asym. or inhomogeneous state of the ArtP dimer bound with triphosphorylated nucleotides. With this investigation, a model system was established for screening all functional states occurring in one ABC transporter in native lipid environment.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
UniBasel Contributors:Engel, Andreas H
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Wiley-VCH
ISSN:1439-4227
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:14 Sep 2012 07:22
Deposited On:14 Sep 2012 07:15

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