Atomic force microscopy of biological membranes

Frederix, P. L. and Bosshart, P. D. and Engel, A.. (2009) Atomic force microscopy of biological membranes. Biophysical journal, Vol. 96, H. 2. pp. 329-338.

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Official URL: http://edoc.unibas.ch/dok/A5262451

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Atomic force microscopy (AFM) is an ideal method to study the surface topography of biological membranes. It allows membranes that are adsorbed to flat solid supports to be raster-scanned in physiological solutions with an atomically sharp tip. Therefore, AFM is capable of observing biological molecular machines at work. In addition, the tip can be tethered to the end of a single membrane protein, and forces acting on the tip upon its retraction indicate barriers that occur during the process of protein unfolding. Here we discuss the fundamental limitations of AFM determined by the properties of cantilevers, present aspects of sample preparation, and review results achieved on reconstituted and native biological membranes.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
UniBasel Contributors:Engel, Andreas H
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Biophysical Society
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:14 Sep 2012 07:22
Deposited On:14 Sep 2012 07:14

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