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Simultaneous separation of intracellular and extracellular lactate NMR signals of human erythrocytes

Kohler, G. and Rost, F. and Seelig, J.. (2007) Simultaneous separation of intracellular and extracellular lactate NMR signals of human erythrocytes. Magnetic Resonance in Medicine, 58 (2). pp. 213-217.

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Official URL: http://edoc.unibas.ch/dok/A5257375

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Abstract

Intracellular/extracellular lactate (Lac) distribution has been determined before in human and animal erythrocytes (red blood cells [RBCs]) with various methods. However, all previous methods determine intra- and extracellular Lac separately or indirectly. Now, (13)C-NMR spectroscopy has been used to monitor intra- and extracellular Lac simultaneously in intact RBCs. Isolated human RBCs were incubated with [3-(13)C]-Lac, [3-(13)C]-pyruvate (Pyr), and [1-(13)C]-glucose (Gluc). A distortionless enhancement by polarization transfer (DEPT) sequence was used (TR = 3.3 s, N = 128) to monitor the (13)C-NMR resonances in both compartments. The intra- and extracellular methyl group resonances of Lac and Pyr were clearly separated by 9.6 Hz and 7.0 Hz, respectively, under normoxic conditions due to the RBC chemical-shift effect. The results show that the chemical-shift effect of RBCs is convenient to monitor intra- and extracellular Lac simultaneously in intact RBCs under normoxic conditions.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Biophysical Chemistry (Seelig J)
UniBasel Contributors:Seelig, Joachim
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Wiley-Liss
ISSN:0740-3194
e-ISSN:1522-2594
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:14 Nov 2017 13:13
Deposited On:22 Mar 2012 13:17

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