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Imaging of vascular smooth muscle cells with soft x-ray spectromicroscopy

Sedlmair, Julia and Gleber, Sophie-Charlotte and Mert, Semra Öztürk and Bertilson, Michael and von Hofsten, Olov and Thieme, Jürgen and Pfohl, Thomas. (2011) Imaging of vascular smooth muscle cells with soft x-ray spectromicroscopy. Microscopy and Microanalysis, Vol. 17, H. 6. pp. 991-1001.

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Official URL: http://edoc.unibas.ch/dok/A5848978

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Abstract

Using X-ray microscopy and spectromicroscopy, vascular smooth muscle cells (VSMCs) were imaged, prepared without using additional embedding material or staining, but by applying simple, noncryo fixation techniques. The cells were imaged with a compact source transmission X-ray microscope and a scanning transmission X-ray microscope (STXM). With the STXM, spectromicroscopy was performed at the C K-edge and the Ca LIII,II-edges. VSMCs were chosen because of their high amount of actin stress fibers, so that the actin cytoskeleton should be visible. Other parts of the cell, such as the nucleus and organelles, were also identified from the micrographs. Both in the spectra and the images, the effects of the different preparation procedures were observable. Furthermore, Ca hotspots were detected and their density is determined.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Biophysikalische Chemie (Pfohl)
UniBasel Contributors:Pfohl, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Springer-Verlag
ISSN:1435-8115
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Sep 2012 07:20
Deposited On:14 Sep 2012 07:02

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