Reuter, R. and Lehner, C. F. and Nigg, E. A. and Lührmann, R.. (1986) A monoclonal antibody specific for snRNPs U1 and U2. FEBS letters, Vol. 201, H. 1. pp. 25-30.
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Official URL: http://edoc.unibas.ch/dok/A5249516
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Abstract
A monoclonal antibody (D-5) is described which selectively precipitates snRNPs U1 and U2. The antibody was derived from a mouse immunized with extracts from chick embryonic nuclei. By immunoblotting on either total proteins from purified snRNPs U1-U6, U2-U6 or U1 only, we could demonstrate that the monoclonal antibody cross-reacts with the U1 RNP specific polypeptide A and the U2 RNP specific polypeptide B", thereby establishing that the two snRNP proteins share at least one epitope. D-5 precipitates snRNPs U1 and U2 from a variety of species, including man, chicken, mouse, rat kangaroo and Xenopus laevis. It will thus be a useful tool for studying structure function relationships of the two snRNP species in different cell systems.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg) |
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| UniBasel Contributors: | Nigg, Erich A. |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Elsevier Science |
| ISSN: | 0014-5793 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Related URLs: | |
| Identification Number: |
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| Last Modified: | 22 Mar 2012 14:19 |
| Deposited On: | 22 Mar 2012 13:17 |
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