Protein decorated membranes by specific molecular interactions

Nehring, Rainer and Palivan, Cornelia G. and Moreno-Flores, Susana and Mantion, Alexandre and Tanner, Pascal and Toca-Herrera, Jose Luis and Thuenemann, Andreas and Meier, Wolfgang. (2010) Protein decorated membranes by specific molecular interactions. Soft Matter, 6 (12). pp. 2815-2824.

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Official URL: http://edoc.unibas.ch/dok/A5841681

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Here we characterize new metal-functionalized amphiphilic diblock copolymers, developed for both surface and soln. mol. recognition applications. Polybutadiene-block-poly(ethylene oxide) copolymers functionalized with nitrilotriacetic acid and tris(nitrilotriacetic acid) were complexed with nickel(II) to obtain coordination sites for oligohistidine residues of model proteins. Mixts. of functionalized polymers with the resp. non-functionalized block copolymers self-assemble in aq. soln. into vesicular structures with a controlled d. of the metal end-groups on their surface. In soln., binding of His6-tagged green fluorescent protein (EGFP) and red fluorescent protein (RFP) to the vesicle surface was quantified by fluorescence correlation spectroscopy. Small-angle X-ray scattering indicates an increase of the membrane thickness by 2-3 nm upon protein binding. Block copolymer monolayers at the air-water interface and on solid support served as a model system to characterize the protein-decorated membranes by Brewster angle microscopy and AFM. High resoln. AFM of solid-supported, hydrated monolayers indicates that the proteins form densely packed and partially ordered arrays with the cylindrically shaped EGFP mols. lying flat on the surface of the films.
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Royal Society of Chemistry
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:04 Jun 2018 11:53
Deposited On:14 Sep 2012 06:58

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