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Solid Peptide Nanoparticles : Structural Characterization and Quantification of Cargo Encapsulation

Dittrich, Christian and Meier, Wolfgang. (2010) Solid Peptide Nanoparticles : Structural Characterization and Quantification of Cargo Encapsulation. Macromolecular bioscience, Vol. 10, H. 12. pp. 1406-1415.

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Official URL: http://edoc.unibas.ch/dok/A5841674

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Abstract

CD3ac, an uncharged and strongly hydrophobic 10 amino acid peptide (Ac-LK(Ac)-LK(Ac)-LK(Ac)-LW-DL-LW-DL-LW-DL-LW-NH2) was synthesized and purified. The peptide readily dissolves in ethanol and - upon solvent exchange to water - assembles into solid spherical particles with diams. of around 500 nm and low size-polydispersity. CD3ac self-assembles in a convenient one-step-process in the absence of a templating two-phase solvent system or any other templating agents. CD reveals a gramicidin-like secondary structure, which can be attributed to the presence of D-leucine, whereas LCD3ac, a peptide of identical constitution yet composed entirely of L-amino acids ppts. amorphously. The unacetylated deriv. of LCD3ac (LCD3) displays ?-helical character in CD. During the process of bead formation, CD3ac can take up and enrich water-sol. and - insol. cargo compds., which is exemplified by the encapsulation of rose bengal (RB) and 5-carboxy-fluorescein (CF), two xanthene derivs. We confirmed their presence in CD3ac beads by confocal fluorescence microscopy and quantified the encapsulation efficiency by absorption measurements of dissolved RB-contg. peptide bead suspensions. Loaded CD3ac beads consist of up to 40 mol-% RB, which corresponds to a logarithmic partition coeff. of 2.95. To the best of our knowledge CD3ac is the first peptide synthesized by Fmoc chem. which forms solid particles in the nano- and micrometer size range and holds promise for drug delivery applications. [on SciFinder(R)]
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Wiley-VCH
ISSN:1616-5195
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Sep 2012 07:19
Deposited On:14 Sep 2012 06:58

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