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A protocol for HPLC-based activity profiling for natural products with activities against tropical parasites

Adams, Michael and Zimmermann, Stefanie and Kaiser, Marcel and Brun, Reto and Hamburger, Matthias. (2009) A protocol for HPLC-based activity profiling for natural products with activities against tropical parasites. Natural Product Communications, 4 (10). pp. 1377-1381.

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Official URL: http://edoc.unibas.ch/dok/A5843122

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Abstract

HPLC based activity profiling is an effective strategy to accelerate the discovery of new hits and leads from nature. It conveniently combines the superior separation power of HPLC micro scale compound separation with miniaturized biological screening methods, and on-line and off-line spectroscopy (PDA, MS(n), HR-MS, NMR) for structure elucidation. We here describe a protocol for the discovery of natural products with antimalarial, antileishmanial and antitrypanosomal activity, from extracts libraries in 96-well format. Analytical gradient HPLC on a 3 x 150 mm column of 350 microg of extract, and collection of one-minute fractions into 96 deep-well microtiter plates, parallel evaporation of the micro-fractions, and a suitable dilution scheme permitted parallel activity profiling against three parasites from a single HPLC injection. The protocol was validated with extracts and positive controls such as Artemisia annua
Faculties and Departments:05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmazeutische Biologie (Hamburger)
UniBasel Contributors:Hamburger, Matthias and Brun, Reto and Wolfer, Sascha and Zimmermann, Stefanie
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Natural Product
ISSN:1934-578X
e-ISSN:1555-9475
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:28 Nov 2017 07:28
Deposited On:14 Sep 2012 06:53

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