Lack of insertional-deletional polymorphism in a collection of Mycobacterium ulcerans isolates from Ghanaian Buruli ulcer patients

Käser, M. and Gutmann, O. and Hauser, J. and Stinear, T. and Cole, S. and Yeboah-Manu D., and Dernick, G. and Certa, U. and Pluschke, G.. (2009) Lack of insertional-deletional polymorphism in a collection of Mycobacterium ulcerans isolates from Ghanaian Buruli ulcer patients. Journal of clinical microbiology, Vol. 47, H. 11. pp. 3640-3646.

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Official URL: http://edoc.unibas.ch/dok/A5843251

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Mycobacterium ulcerans causes the devastating infectious skin disease Buruli ulcer and has a monomorphic population structure. The resolution of conventional genetic fingerprinting methods is therefore not sufficient for micro-epidemiological studies aiming to characterize transmission pathways. In a previous comparative genomic hybridisation analysis with a microarray covering part of the M. ulcerans genome, we have found insertion sequence-associated extensive insertional-deletional sequence polymorphisms among M. ulcerans isolates of diverse geographic origin that allowed distinguishing between strains coming from different continents. Since large numbers of insertion sequences are spread over the genome of African M. ulcerans strains, we reasoned that these may drive large sequence polymorphism in otherwise clonal local mycobacterial populations. In this study, we used a printed DNA microarray covering the whole genome of the Ghanaian M. ulcerans reference strain Agy99 for comparative genomic hybridisation. The assay identified multiple regions of difference when DNA of a Japanese M. ulcerans strain was analyzed. In contrast, not a single insertional/deletional genomic variation was found within a panel of disease isolates coming from a Buruli ulcer endemic area of Ghana. These results indicate that - despite the expectations deduced from other mycobacterial pathogens - only analyses of single nucleotide polymorphisms will have the potential to differentiate local populations of M. ulcerans
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Molecular Immunology (Pluschke)
UniBasel Contributors:Pluschke, Gerd and Käser, Michael
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Sep 2012 07:18
Deposited On:14 Sep 2012 06:43

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