edoc

DNA-binding characteristics of cnidarian Pax-C and Pax-B proteins in vivo and in vitro : no simple relationship with the Pax-6 and Pax-2/5/8 classes

Plaza, S. and De Jong, D. M. and Gehring, W. J. and Miller, D. J.. (2003) DNA-binding characteristics of cnidarian Pax-C and Pax-B proteins in vivo and in vitro : no simple relationship with the Pax-6 and Pax-2/5/8 classes. Journal of experimental zoology. Part B, Molecular and developmental evolution, Vol. 299, H. 1. pp. 26-35.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5257285

Downloads: Statistics Overview

Abstract

Cnidarians are the simplest animals in which distinct eyes are present. We have previously suggested that cnidarian Pax-Cam might represent a precursor of the Pax-6 class. Here we show that when expressed in Drosophila imaginal discs, Pax-Cam chimeric proteins containing the C-terminal region of EY were capable of eye induction and driving expression of a reporter gene under the control of a known EY target (the sine oculis gene). Whilst these results are consistent with a Pax-6-like function for Pax-Cam, in band shift experiments we were unable to distinguish the DNA-binding behaviour of the Pax-Cam Paired domain from that of a second Acropora Pax protein, Pax-Bam. The ability of a Pax-Bam/EY chimera to also induce eye formation in leg imaginal discs, together with the in vitro data, cast doubt on previously assumed direct relationships between cnidarian Pax genes and the Pax-6 and Pax-2/5/8 classes of bilateral animals.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Gehring)
UniBasel Contributors:Gehring, Walter Jakob
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley-Liss
ISSN:1552-5007
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:19
Deposited On:22 Mar 2012 13:17

Repository Staff Only: item control page