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The Plk1-dependent phosphoproteome of the early mitotic spindle

Santamaria, A. and Wang, B. and Elowe, S. and Malik, R. and Zhang, F. and Bauer, M. and Schmidt, A. and Sillje, H. H. and Koerner, R. and Nigg, E. A.. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Molecular & cellular proteomics, Vol. 10, H. 1 , M110.004457.

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Official URL: http://edoc.unibas.ch/dok/A5844173

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Abstract

Polo-like kinases regulate many aspects of mitotic and meiotic progression from yeast to man. Inearly mitosis, mammalian Polo-like kinase 1 (Plk1) controls centrosome maturation, spindleassembly and microtubule attachment to kinetochores. However, despite the essential and diversefunctions of Plk1, the full range of Plk1 substrates remains to be explored. To investigate thePlk1-dependent phosphoproteome of the human mitotic spindle, we combine SILAC with Plk1inactivation or depletion, followed by spindle isolation and mass spectrometry. Our studyidentifies 358 unique Plk1-dependent phosphorylation sites on spindle proteins, including novelsubstrates, illustrating the complexity of the Plk1-dependent signaling network. Over 100 siteswere validated by in vitro phosphorylation of peptide arrays, resulting in a broadening of the Plk1consensus motif. Collectively our data provides a rich source of information on Plk1-dependentphosphorylation, Plk1 docking to substrates, the influence of phosphorylation on proteinlocalization, and the functional interaction between Plk1 and Aurora A on the early mitoticspindle.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg)
05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Proteomics (Schmidt)
UniBasel Contributors:Nigg, Erich A. and Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:The American Society for Biochemistry and Molecular Biology
ISSN:1535-9484
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Sep 2012 07:17
Deposited On:14 Sep 2012 06:40

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