Plasmodium berghei ANKA: selection of resistance to piperaquine and lumefantrine in a mouse model

Kiboi, D. M. and Irungu, B. N. and Langat, B. and Wittlin, S. and Brun, R. and Chollet, J. and Abiodun, O. and Nganga, J. K. and Nyambati, V. C. and Rukunga, G. M. and Bell, A. and Nzila, A.. (2009) Plasmodium berghei ANKA: selection of resistance to piperaquine and lumefantrine in a mouse model. Experimental parasitology : EP, Vol. 122, H. 3. pp. 196-202.

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Official URL: http://edoc.unibas.ch/dok/A5843294

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We have selected piperaquine (PQ) and lumefantrine (LM) resistant Plasmodium berghei ANKA parasite lines in mice by drug pressure. Effective doses that reduce parasitaemia by 90% (ED(90)) of PQ and LM against the parent line were 3.52 and 3.93 mg/kg, respectively. After drug pressure (more than 27 passages), the selected parasite lines had PQ and LM resistance indexes (I(90)) [ED(90) of resistant line/ED(90) of parent line] of 68.86 and 63.55, respectively. After growing them in the absence of drug for 10 passages and cryo-preserving them at -80 degrees C for at least 2 months, the resistance phenotypes remained stable. Cross-resistance studies showed that the PQ-resistant line was highly resistant to LM, while the LM-resistant line remained sensitive to PQ. Thus, if the mechanism of resistance is similar in P. berghei and Plasmodium falciparum, the use of LM (as part of Coartem) should not select for PQ resistance.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Parasite Chemotherapy (Mäser)
UniBasel Contributors:Brun, Reto and Wittlin, Sergio
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Sep 2012 07:17
Deposited On:14 Sep 2012 06:39

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