Fluorescent fusion protein knockout mediated by anti-GFP nanobody

Caussinus, Emmanuel and Kanca, Oguz and Affolter, Markus. (2012) Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Nature structural & molecular biology, Vol. 19, no. 1 , S. 117–121.

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Official URL: http://edoc.unibas.ch/dok/A5848701

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The use of genetic mutations to study protein functions in vivo is a central paradigm of modern biology. Recent advances in reverse genetics such as RNA interference and morpholinos are widely used to further apply this paradigm. Nevertheless, such systems act upstream of the proteic level, and protein depletion depends on the turnover rate of the existing target proteins. Here we present deGradFP, a genetically encoded method for direct and fast depletion of target green fluorescent protein (GFP) fusions in any eukaryotic genetic system. This method is universal because it relies on an evolutionarily highly conserved eukaryotic function, the ubiquitin pathway. It is traceable, because the GFP tag can be used to monitor the protein knockout. In many cases, it is a ready-to-use solution, as GFP protein-trap stock collections are being generated in Drosophila melanogaster and in Danio rerio.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Affolter)
UniBasel Contributors:Affolter, Markus
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publ. Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Mar 2013 11:06
Deposited On:14 Sep 2012 06:38

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