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Distinct cellular mechanisms of blood vessel fusion in the zebrafish embryo

Herwig, Lukas and Blum, Yannick and Krudewig, Alice and Ellertsdottir, Elin and Lenard, Anna and Belting, Heinz-Georg and Affolter, Markus. (2011) Distinct cellular mechanisms of blood vessel fusion in the zebrafish embryo. Current biology, Vol. 21, H. 22. pp. 1942-1948.

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Official URL: http://edoc.unibas.ch/dok/A5848360

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Abstract

Although many of the cellular and molecular mechanisms of angiogenesis have been intensely studied [ 1], little is known about the processes that underlie vascular anastomosis. We have generated transgenic fish lines expressing an EGFP- tagged version of the junctional protein zona occludens 1 (ZO1) to visualize individual cell behaviors that occur during vessel fusion and lumen formation in vivo. These live observations show that endothelial cells (ECs) use two distinct morphogenetic mechanisms, cell membrane invagination and cord hollowing to generate different types of vascular tubes. During initial steps of anastomosis, cell junctions that have formed at the initial site of cell contacts expand into rings, generating a cellular interface of apical membrane compartments, as defined by the localization of the apical marker podocalyxin-2 (Pdxl2). During the cord hollowing process, these apical membrane compartments are brought together via cell rearrangements and extensive junctional remodeling, resulting in lumen coalescence and formation of a multicellular tube. Vessel fusion by membrane invagination occurs adjacent to a preexisting lumen in a proximal to distal direction and is blood-flow dependent. Here, the invaginating inner cell membrane undergoes concomitant apicobasal polarization and the vascular lumen is formed by the extension of a transcellular lumen through the EC, which forms a unicellular or seamless tube.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Affolter)
UniBasel Contributors:Affolter, Markus
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Cell Press
ISSN:0960-9822
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:19 Jul 2013 07:42
Deposited On:14 Sep 2012 06:38

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