edoc

High-resolution low-dose scanning transmission electron microscopy

Buban, James P. and Ramasse, Quentin and Gipson, Bryant and Browning, Nigel D. and Stahlberg, Henning. (2010) High-resolution low-dose scanning transmission electron microscopy. Journal of electron microscopy, Vol. 59, no. 2 , S. 103–112.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5262361

Downloads: Statistics Overview

Abstract

During the past two decades instrumentation in scanning transmission electron microscopy (STEM) has pushed toward higher intensity electron probes to increase the signal-to-noise ratio of recorded images. While this is suitable for robust specimens, biological specimens require a much reduced electron dose for high-resolution imaging. We describe here protocols for low-dose STEM image recording with a conventional field-emission gun STEM, while maintaining the high-resolution capability of the instrument. Our findings show that a combination of reduced pixel dwell time and reduced gun current can achieve radiation doses comparable to low-dose TEM.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Japanes Society of Electron Microscopy
ISSN:0022-0744
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:14 Sep 2012 07:17
Deposited On:14 Sep 2012 06:38

Repository Staff Only: item control page