Defects in the Secretory Pathway and High Ca2+ Induce Multiple P-bodies

Kilchert, C. and Weidner, J. and Prescianotto-Baschong, C. and Spang, A.. (2010) Defects in the Secretory Pathway and High Ca2+ Induce Multiple P-bodies. Molecular Biology of the Cell, 21 (15). pp. 2624-2638.

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Monitoring Editor: Reid Gilmore mRNA is sequestered and turned over in cytoplasmic processing bodies (PBs), which are induced by various cellular stresses. Unexpectedly, in S. cerevisiae, mutants of the small GTPase Arf1 and various secretory pathway mutants induced a significant increase in PB number, as compared with PB induction by starvation or oxidative stress. Exposure of wild-type cells to osmotic stress or high extracellular Ca(2+) mimicked this increase in PB number. Conversely, intracellular Ca(2+)-depletion strongly reduced PB formation in the secretory mutants. In contrast to PB induction through starvation or osmotic stress, PB formation in secretory mutants and by Ca(2+) required the PB components Pat1 and Scd6, and calmodulin, indicating that different stressors act through distinct pathways. Consistent with this hypothesis, when stresses were combined, PB number did not correlate with the strength of the translational block, but rather with the type of stress encountered. Interestingly, independent of the stressor, PBs appear as spheres of approximately 40-100 nm connected to the endoplasmic reticulum (ER), consistent with the idea that translation and silencing/degradation occur in a spatially coordinated manner at the ER. We propose that PB assembly in response to stress occurs at the ER and depends on intracellular signals that regulate PB number.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spang)
UniBasel Contributors:Spang, Anne
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Society for Cell Biology
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:08 Nov 2017 11:00
Deposited On:14 Sep 2012 06:37

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