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Flexible Accelerated STOP Tetracycline Operator-knockin (FAST) : a versatile and efficient new gene modulating system

Tanaka, K. F. and Ahmari, S. E. and Leonardo, E. D. and Richardson-Jones, J. W. and Budreck, E. C. and Scheiffele, P. and Sugio, S. and Inamura, N. and Ikenaka, K. and Hen, R.. (2010) Flexible Accelerated STOP Tetracycline Operator-knockin (FAST) : a versatile and efficient new gene modulating system. Biological Psychiatry, Vol. 67, H. 8. pp. 770-773.

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Official URL: http://edoc.unibas.ch/dok/A5842494

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Abstract

We created the Flexible Accelerated STOP Tetracycline Operator (tetO)-knockin (FAST) system, an efficient method for manipulating gene expression in vivo to rapidly screen animal models of disease. A single gene targeting event yields two distinct knockin mice-STOP-tetO and tetO knockin-that permit generation of multiple strains with variable expression patterns: 1) knockout, 2) Cre-mediated rescue, 3) tetracycline-controlled transcriptional activator (tTA)-mediated misexpression, 4) tetracycline-controlled transcriptional activator (tTA)-mediated overexpression, and 5) tetracycline-controlled transcriptional silencer (tTS)-mediated conditional knockout/knockdown. Using the FAST system, multiple gain-of-function and loss-of-function strains can therefore be generated on a time scale not previously achievable. These strains can then be screened for clinically relevant abnormalities. We demonstrate the flexibility and broad applicability of the FAST system by targeting several genes encoding proteins implicated in neuropsychiatric disorders: Mlc1, neuroligin 3, the serotonin 1A receptor, and the serotonin 1B receptor.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Neurobiology > Cell Biology (Scheiffele)
UniBasel Contributors:Scheiffele, Peter
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0006-3223
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Sep 2012 07:17
Deposited On:14 Sep 2012 06:37

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