A non-coding antisense RNA in tie-1 locus regulates tie-1 function in vivo

Li, K. and Blum, Y. and Verma, A. and Liu, Z. and Pramanik, K. and Leigh, N. R. and Chun, C. Z. and Samant, G. V. and Zhao, B. and Garnaas, M. K. and Horswill, M. A. and Affolter, M. and Stanhope, S. A. and North, P. E. and Miao, R. Q. and Wilkinson, G. A. and Ramchandran, R.. (2009) A non-coding antisense RNA in tie-1 locus regulates tie-1 function in vivo. Blood, Vol. 115, No. 1. pp. 133-139.

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Official URL: http://edoc.unibas.ch/dok/A5258891

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Recently, messenger RNAs in eukaryotes have shown to associate with antisense (AS) transcript partners that are often referred to as long noncoding RNAs (lncRNAs) whose function is largely unknown. Here, we have identified a natural AS transcript for tyrosine kinase containing immunoglobulin and epidermal growth factor homology domain-1 (tie-1), tie-1AS lncRNA in zebrafish, mouse and humans. In embryonic zebrafish, tie-1AS lncRNA transcript is expressed temporally and spatially in vivo with its native target, the tie-1 coding transcript and in additional locations (ear & brain). The tie-1AS lncRNA selectively binds tie-1 mRNA in vivo and regulates tie-1 transcript levels resulting in specific defects in endothelial cell contact junctions in vivo and in vitro. The ratio of tie-1 vs. tie-1AS lncRNA is altered in human vascular anomaly samples. These results directly implicate non-coding RNA mediated transcriptional regulation of gene expression as a fundamental control mechanism for physiological process such as vascular development.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Affolter)
UniBasel Contributors:Affolter, Markus
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:08 Jun 2012 06:56
Deposited On:08 Jun 2012 06:49

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