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Amyloid structure and assembly : insights from scanning transmission electron microscopy

Goldsbury, C. and Baxa, U. and Simon, M. N. and Steven, A. C. and Engel, A. and Wall, J. S. and Aebi, U. and Muller, S. A.. (2011) Amyloid structure and assembly : insights from scanning transmission electron microscopy. Journal of structural biology, Vol. 173, H. 11-13. pp. 1-13.

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Official URL: http://edoc.unibas.ch/dok/A5842517

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Abstract

Amyloid fibrils are filamentous protein aggregates implicated in several common diseases such as Alzheimer`s disease and type II diabetes. Similar structures are also the molecular principle of the infectious spongiform encephalopathies such as Creutzfeldt-Jakob disease in humans, scrapie in sheep, and of the so-called yeast prions, inherited non-chromosomal elements found in yeast and fungi. Scanning transmission electron microscopy (STEM) is often used to delineate the assembly mechanism and structural properties of amyloid aggregates. In this review we consider specifically contributions and limitations of STEM for the investigation of amyloid assembly pathways, fibril polymorphisms and structural models of amyloid fibrils. This type of microscopy provides the only method to directly measure the mass-per-length (MPL) of individual filaments. Made on both in vitro assembled and ex vivo samples, STEM mass measurements have illuminated the hierarchical relationships between amyloid fibrils and revealed that polymorphic fibrils and various globular oligomers can assemble simultaneously from a single polypeptide. The MPLs also impose strong constraints on possible packing schemes, assisting in molecular model building when combined with high-resolution methods like solid-state nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR).
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Aebi)
05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
UniBasel Contributors:Engel, Andreas H and Aebi, Ueli
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Academic Press
ISSN:1047-8477
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:11 Oct 2012 15:16
Deposited On:08 Jun 2012 06:49

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