edoc

The fold of alpha-synuclein fibrils

Vilar, Marçal and Chou, Hui-Ting and Lührs, Thorsten and Maji, Samir K. and Riek-Loher, Dominique and Verel, Rene and Manning, Gerard and Stahlberg, Henning and Riek, Roland. (2008) The fold of alpha-synuclein fibrils. Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, H. 25. pp. 8637-8642.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5842549

Downloads: Statistics Overview

Abstract

The aggregation of proteins into amyloid fibrils is associated with several neurodegenerative diseases. In Parkinson's disease it is believed that the aggregation of alpha-synuclein (alpha-syn) from monomers by intermediates into amyloid fibrils is the toxic disease-causative mechanism. Here, we studied the structure of alpha-syn in its amyloid state by using various biophysical approaches. Quenched hydrogen/deuterium exchange NMR spectroscopy identified five beta-strands within the fibril core comprising residues 35-96 and solid-state NMR data from amyloid fibrils comprising the fibril core residues 30-110 confirmed the presence of beta-sheet secondary structure. The data suggest that beta1-strand interacts with beta2, beta2 with beta3, beta3 with beta4, and beta4 with beta5. High-resolution cryoelectron microscopy revealed the protofilament boundaries of approximately 2 x 3.5 nm. Based on the combination of these data and published structural studies, a fold of alpha-syn in the fibrils is proposed and discussed.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:National Academy of Sciences
ISSN:0027-8424
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:08 Jun 2012 06:56
Deposited On:08 Jun 2012 06:47

Repository Staff Only: item control page