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Evaluation des VNTR (variable number of tandem repeats) des isolats de Mycobacterium bovis en Algérie

Sahraoui, N. and Muller, B. and Djamel, Y. and Fadela, B. and Rachid, O. and Zinsstag, J. and Djamel, G.. (2010) Evaluation des VNTR (variable number of tandem repeats) des isolats de Mycobacterium bovis en Algérie. Annales de Biologie Clinique, Vol. 68, H. 4. pp. 449-453.

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Official URL: http://edoc.unibas.ch/dok/A5842889

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Abstract

The discriminatory potency of variable number of tandem repeats (VNTR), based on 7 loci (MIRU 26, 27 and 5 ETRs A, B, C, D, E) was assayed on Mycobacterium bovis strains obtained from samples due to tuberculosis in two slaughterhouses in Algeria. The technique of MIRU-VNTR has been evaluated on 88 strains of M. bovis and one strain of M. caprea and shows 41 different profiles. Results showed that the VNTR were highly discriminatory with an allelic diversity of 0.930 when four loci (ETR A, B, C and MIRU 27) were highly discriminatory (h < 0.25) and three loci (ETR D and E MIRU 26) moderately discriminatory (0.11 > h > 0.25). The four VNTR loci were highly discriminatory be adequate for the first proper differentiation of strains of M. bovis in Algeria. The VNTR technique has proved a valuable tool for further development and application of epidemiological research for the of tuberculosis transmission in Algeria
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Human and Animal Health > One Health (Zinsstag)
UniBasel Contributors:Zinsstag, Jakob Z
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Libbey
ISSN:0003-3898
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:08 Jun 2012 06:55
Deposited On:08 Jun 2012 06:46

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