Label-free detection of single protein molecules and protein-protein interactions using synthetic nanopores

Han, Anpan and Creus, Marc and Schuermann, Gregor and Linder, Vincent and Ward, Thomas R. and de Rooij, Nico F. and Staufer, Urs. (2008) Label-free detection of single protein molecules and protein-protein interactions using synthetic nanopores. Analytical Chemistry, 80 (12). pp. 4651-4658.

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Official URL: http://edoc.unibas.ch/dok/A5254448

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Nanofabricated pores in 20 nm-thick silicon nitride membranes were used to probe various protein analytes as well as to perform an antigen−antibody binding assay. A two-compartment electrochemical cell was separated by a single nanopore, 28 nm in diameter. Adding proteins to one compartment caused current perturbations in the ion current flowing through the pore. These perturbations correlated with both the charge and the size of the protein or of a protein−protein complex. The potential of this nanotechnology for studying protein−protein interactions is highlighted with the sensitive detection of β-human chorionic gonadotropin, a hormone and clinical biomarker of pregnancy, by monitoring in real time and at a molecular level the formation of a complex between hormones and antibodies in solution. In this form, the assay compared advantageously to immunoassays, with the important difference that labels, immobilization, or amplification steps were no longer needed. In conclusion, we present proof-of-principle that properties of proteins and their interactions can be investigated in solution using synthetic nanopores and that these interactions can be exploited to measure protein concentrations accurately.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)
UniBasel Contributors:Ward, Thomas R. R.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:24 Apr 2017 11:57
Deposited On:22 Mar 2012 14:15

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