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Peptic and tryptic digestion of peptides and proteins monitored by capillary electrophoresis with contactless conductivity detection

Schuchert-Shi, A. and Hauser, P. C.. (2009) Peptic and tryptic digestion of peptides and proteins monitored by capillary electrophoresis with contactless conductivity detection. Analytical Biochemistry, Vol. 387, H. 2. pp. 202-207.

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Official URL: http://edoc.unibas.ch/dok/A5251979

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Abstract

The feasibility of monitoring the peptic and tryptic digestion of peptides and proteins with capillary electrophoresis using contactless conductivity detection was investigated. The peptide minigastrin I and the proteins cytochrome c from bovine heart, human serum albumin (HSA), myoglobin, and bovine serum albumin (BSA) were digested off-line with pepsin, and the resulting peptide and amino acid fragments were successfully separated and detected by conductivity measurement. Cytochrome c and myoglobin were also subjected to off-line cleavage with trypsin. On-line digestion using the electrophoretically mediated microanalysis (EMMA) approach was demonstrated with cytochrome c and apomyoglobin using trypsin. (C) 2009 Elsevier Inc. All rights reserved.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
UniBasel Contributors:Hauser, Peter C. P. C.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0003-2697
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:22 Mar 2012 14:29
Deposited On:22 Mar 2012 14:10

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