edoc

Melav2, an elav-like gene, is essential for spermatid differentiation in the flatworm Macrostomum lignano

Sekii, K. and Salvenmoser, W. and De Mulder, K. and Schärer, L. and Ladurner, P.. (2009) Melav2, an elav-like gene, is essential for spermatid differentiation in the flatworm Macrostomum lignano. BMC developmental biology, Vol. 9 , 62.

[img]
Preview
PDF - Published Version
Available under License CC BY (Attribution).

4Mb

Official URL: http://edoc.unibas.ch/dok/A5257261

Downloads: Statistics Overview

Abstract

Background Failure of sperm differentiation is one of the major causes of male sterility. During spermiogenesis, spermatids undergo a complex metamorphosis, including chromatin condensation and cell elongation. Although the resulting sperm morphology and property can vary depending on the species, these processes are fundamental in many organisms. Studying genes involved in such processes can thus provide important information for a better understanding of spermatogenesis, which might be universally applied to many other organisms. Results In a screen for genes that have gonad-specific expression we isolated an elav-like gene, melav2, from Macrostomum lignano, containing the three RNA recognition motifs characteristic of elav-like genes. We found that melav2 mRNA was expressed exclusively in the testis, as opposed to the known elav genes, which are expressed in the nervous system. The RNAi phenotype of melav2 was characterized by an aberrant spermatid morphology, where sperm elongation often failed, and an empty seminal vesicle. Melav2 RNAi treated worms were thus male-sterile. Further analysis revealed that in melav2 RNAi treated worms precocious chromatin condensation occurred during spermatid differentiation, resulting in an abnormally tightly condensed chromatin and large vacuoles in round spermatids. In addition, immunostaining using an early-spermatid specific antibody revealed that melav2 RNAi treated worms had a larger amount of signal positive cells, suggesting that many cells failed the transition from early spermatid stage. Conclusions We characterize a new function for elav-like genes, showing that melav2 plays a crucial role during spermatid differentiation, especially in the regulation of chromatin condensation and/or cell elongation.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Zoologie > Evolutionary Biology (Schärer)
UniBasel Contributors:Sekii, Kiyono and Schärer, Lukas
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:BioMed Central
ISSN:1471-213X
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Last Modified:31 Dec 2015 10:47
Deposited On:22 Mar 2012 14:05

Repository Staff Only: item control page