Quantitative time-resolved measurement of membrane protein-ligand interactions using microcantilever array sensors

Braun, Thomas and Ghatkesar, Murali Krishna and Backmann, Natalija and Grange, Wilfried and Boulanger, Pascale and Letellier, Lucienne and Lang, Hans-Peter and Bietsch, Alex and Gerber, Christoph and Hegner, Martin. (2009) Quantitative time-resolved measurement of membrane protein-ligand interactions using microcantilever array sensors. Nature Nanotechnology, 4 (3). pp. 179-185.

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Official URL: http://edoc.unibas.ch/dok/A5251972

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Membrane proteins are central to many biological processes, and the Interactions between transmembrane protein receptors and their ligands are of fundamental importance in medical research. However, measuring and characterizing these interactions Is challenging. Here we report that sensors based on arrays of resonating microcantilevers can measure such interactions under physiological conditions. A protein receptor-the FhuA receptor of Escherichia coli-is crystallized in liposomes, and the proteoliposomes then immobilized on the chemically activated gold-coated surface of the sensor by ink-jet spotting in a humid environment, thus keeping the receptors functional. Quantitative mass-binding measurements of the bacterial virus T5 at subpicomolar concentrations are performed. These experiments demonstrate the potential of resonating microcantilevers for the specific, label-free and time-resolved detection of membrane protein-ligand interactions in a micro-array format.
Faculties and Departments:05 Faculty of Science > Departement Physik > Physik > Nanomechanik (Meyer)
05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Braun)
05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Stahlberg)
UniBasel Contributors:Lang, Hans Peter and Braun, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Oct 2021 10:26
Deposited On:22 Mar 2012 13:57

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