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Gd-BOPTA transport into rat hepatocytes : pharmacokinetic analysis of dynamic magnetic resonance images using a hollow-fiber bioreactor

Planchamp, Corinne and Gex-Fabry, Marianne and Dornier, Christophe and Quadri, Rafael and Reist, Marianne and Ivancevic, Marko K. and Vallée, Jean-Paul and Pochon, Sibylle and Terrier, François and Balant, Luc and Stieger, Bruno and Meier, Peter J. and Pastor, Catherine M.. (2004) Gd-BOPTA transport into rat hepatocytes : pharmacokinetic analysis of dynamic magnetic resonance images using a hollow-fiber bioreactor. Investigative radiology, Vol. 39, H. 8. S. 506-515.

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Official URL: http://edoc.unibas.ch/dok/A5261602

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Abstract

RATIONALE AND OBJECTIVES: To investigate the transport of the hepatobiliary magnetic resonance (MR) imaging contrast agent Gd-BOPTA into rat hepatocytes. MATERIALS AND METHODS: In a MR-compatible hollow-fiber bioreactor containing hepatocytes, MR signal intensity was measured over time during the perfusion of Gd-BOPTA. For comparison, the perfusion of an extracellular contrast agent (Gd-DTPA) was also studied. A compartmental pharmacokinetic model was developed to describe dynamic signal intensity-time curves. RESULTS: The dynamic signal intensity-time curves of the hepatocyte hollow-fiber bioreactor during Gd-BOPTA perfusion were adequately fitted by 2 compartmental models. Modeling permitted to discriminate between the behaviors of the extracellular contrast agent (Gd-DTPA) and the hepatobiliary contrast agent (Gd-BOPTA). It allowed the successfully quantification of the parameters involved in such differences. Gd-BOPTA uptake was saturable at high substrate concentrations. CONCLUSIONS: The transport of Gd-BOPTA into rat hepatocytes was successfully described by compartmental analysis of the signal intensity recorded over time and supported the hypothesis of a transporter-mediated uptake.
Faculties and Departments:11 Rektorat und Verwaltung > Vizerektorat Forschung
UniBasel Contributors:Meier-Abt, Peter J.
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Lippincott Williams & Wilkins
ISSN:0020-9996
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:22 Mar 2012 14:24
Deposited On:22 Mar 2012 13:42

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