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Anion transport in basolateral (sinusoidal) liver plasma-membrane vesicles of the little skate (Raja erinacea)

Hugentobler, G. and Fricker, G. and Boyer, J. L. and Meier, P. J.. (1987) Anion transport in basolateral (sinusoidal) liver plasma-membrane vesicles of the little skate (Raja erinacea). The biochemical journal, Vol. 247, Pt. 3. S. 589-595.

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Official URL: http://edoc.unibas.ch/dok/A5261794

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Abstract

The mechanism(s) of [35S]sulphate transport was investigated in basolateral liver plasma-membrane vesicles of the little skate elasmobranch, Raja erinacea. Imposition of an intravesicular alkaline pH gradient (pH 8.0 in/pH 6.0 out) stimulated sulphate uptake 5-10-fold compared with pH-equilibrated (pH 8.0 in = out) conditions and 2-3-fold over equilibrium sulphate uptake (overshoot). This pH-gradient-stimulated sulphate uptake was temperature-dependent, saturable with increasing concentrations of sulphate and could be inhibited by the protonophore carbonyl cyanide m-chlorophenylhydrazone and the anion-transport inhibitors 4,4'-di-isothiocyanostilbene-2,2'-disulphonic acid (DIDS) and probenecid, cis-Inhibition of pH-gradient-driven sulphate uptake was observed with sulphate, oxalate, cholate and bromosulphophthalein, but not with chloride and taurocholate. In addition, sulphate and oxalate trans-stimulated [35S]sulphate uptake under pH-equilibrated conditions. Although also stimulated by an inside-alkaline pH gradient, transmembrane transport of [3H]cholate was not inhibited by DIDS, suggesting that its pH-gradient-driven uptake is not mediated by an anion-transport 'carrier'. In conclusion, these studies indicate that a basolateral plasma-membrane sulphate-transport system has evolved in skate hepatocytes and is similar to that in mammalian liver cells. This archaic anion-exchange system co-transports certain organic anions such as oxalate and has developed early in vertebrate evolution.
Faculties and Departments:11 Rektorat und Verwaltung > Vizerektorat Forschung
UniBasel Contributors:Meier-Abt, Peter J.
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Portland Press
ISSN:0264-6021
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:22 Mar 2012 14:24
Deposited On:22 Mar 2012 13:37

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