A nor-diterpene from Salvia sahendica leaves

Phytochemical investigation of n -hexane extract of Salvia sahendica by normal phase column chromatography resulted in the isolation of six compounds. Structures were established by 1D and 2D NMR spectroscopy, and HRMS, as a new norditerpene 1 , and known terpenoids, sclareol ( 2 ), oleanolic acid ( 3 ), β-sitosterol ( 4 ), salvigenin ( 5 ) and 3 α -hydroxy-11 α ,12 α -epoxyoleanan-28,13 β -olide ( 6 ). The absolute configuration of 1 was confirmed by a combination of X-ray single crystal analysis and electronic circular dichroism spectroscopy. In vitro cytotoxic activity on breast cancer cell line (MDA-MB-231) and also the antimicrobial activity of the pure compounds were tested against Staphylococcus aureus , Bacilus cereus and Escherichia coli.