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Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin

Keller, W. and Crouch, R.. (1972) Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin. Proceedings of the National Academy of Sciences of the United States of America, Vol. 69, H. 11. S. 3360-3364.

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Official URL: http://edoc.unibas.ch/dok/A5258021

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Abstract

Ribonuclease H from human KB cells, chick embryos, calf thymus, avian myeloblastosis virus, and Rous associated virus specifically degrades the RNA of DNA.RNA hybrids, producing mono- and oligoribonucleotides terminated in 5'-phosphates. The cellular RNase H is an endonuclease, whereas the viral enzyme appears to be an exonuclease. Viral DNA polymerase and RNase H copurify through all separation steps. Therefore, RNase H activity is an intrinsic part of the viral DNA polymerase. DNA.RNA hybrids are also degraded by nucleases associated with cellular DNA polymerases and by exonuclease III. However, these nucleases differ from RNase H in their ability to degrade both strands of DNA.RNA hybrids.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Keller)
UniBasel Contributors:Keller, Walter
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:National Academy of Sciences
ISSN:0027-8424
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:23
Deposited On:22 Mar 2012 13:32

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