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In vitro activity of anti-malarial ozonides against an artemisinin-resistant isolate

Baumgärtner, Fabian and Jourdan, Joëlle and Scheurer, Christian and Blasco, Benjamin and Campo, Brice and Mäser, Pascal and Wittlin, Sergio. (2017) In vitro activity of anti-malarial ozonides against an artemisinin-resistant isolate. Malaria journal, 16. p. 45.

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Abstract

Recently published data suggest that artemisinin derivatives and synthetic peroxides, such as the ozonides OZ277 and OZ439, have a similar mode of action. Here the cross-resistance of OZ277 and OZ439 and four additional next-generation ozonides was probed against the artemisinin-resistant clinical isolate Plasmodium falciparum Cam3.I, which carries the K13-propeller mutation R539T (Cam3.I(R539T)).; The previously described in vitro ring-stage survival assay (RSA0-3h) was employed and a simplified variation of the original protocol was developed.; At the pharmacologically relevant concentration of 700 nM, all six ozonides were highly effective against the dihydroartemisinin-resistant P. falciparum Cam3.I(R539T) parasites, showing a per cent survival ranging from <0.01 to 1.83%. A simplified version of the original RSA0-3h method was developed and gave similar results, thus providing a practical drug discovery tool for further optimization of next-generation anti-malarial peroxides.; The absence of in vitro cross-resistance against the artemisinin-resistant clinical isolate Cam3.I(R539T) suggests that ozonides could be effective against artemisinin-resistant P. falciparum. How this will translate to the human situation in clinical settings remains to be investigated.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
UniBasel Contributors:Scheurer, Christian and Mäser, Pascal and Wittlin, Sergio
Item Type:Article, refereed
Publisher:BioMed Central
ISSN:1475-2875
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:25 Apr 2017 07:46
Deposited On:25 Apr 2017 07:46

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