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Environmental and mucosal microbiota and their role in childhood asthma

Birzele, L. T. and Depner, M. and Ege, M. J. and Engel, M. and Kublik, S. and Bernau, C. and Loss, G. J. and Genuneit, J. and Horak, E. and Schloter, M. and Braun-Fahrländer, C. and Danielewicz, H. and Heederik, D. and von Mutius, E. and Legatzki, A.. (2017) Environmental and mucosal microbiota and their role in childhood asthma. Allergy, 72 (1). pp. 109-119.

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Official URL: http://edoc.unibas.ch/54420/

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Abstract

High microbial diversity in the environment has been associated with lower asthma risk, particularly in children exposed to farming. It remains unclear whether this effect operates through an altered microbiome of the mucosal surfaces of the airways.; DNA from mattress dust and nasal samples of 86 school age children was analyzed by 454 pyrosequencing of the 16S rRNA gene fragments. Based on operational taxonomic units (OTUs), bacterial diversity and composition were related to farm exposure and asthma status.; Farm exposure was positively associated with bacterial diversity in mattress dust samples as determined by richness (P = 8.1 × 10(-6) ) and Shannon index (P = 1.3 × 10(-5) ). Despite considerable agreement of richness between mattress and nasal samples, the association of richness with farming in nasal samples was restricted to a high gradient of farm exposure, that is, exposure to cows and straw vs no exposure at all. In mattress dust, the genera Clostridium, Facklamia, an unclassified genus within the family of Ruminococcaceae, and six OTUs were positively associated with farming. Asthma was inversely associated with richness [aOR = 0.48 (0.22-1.02)] and Shannon index [aOR = 0.41 (0.21-0.83)] in mattress dust and to a lower extent in nasal samples [richness aOR 0.63 = (0.38-1.06), Shannon index aOR = 0.66 (0.39-1.12)].; The stronger inverse association of asthma with bacterial diversity in mattress dust as compared to nasal samples suggests microbial involvement beyond mere colonization of the upper airways. Whether inhalation of metabolites of environmental bacteria contributes to this phenomenon should be the focus of future research.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Environmental Exposures and Health > Physical Hazards and Health (Röösli)
UniBasel Contributors:Braun-Fahrländer, Charlotte and Loss, Georg
Item Type:Article, refereed
Publisher:Munksgaard
ISSN:0105-4538
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:24 Apr 2017 13:50
Deposited On:24 Apr 2017 13:50

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